Monoclonal Hybridoma Autoantibodies
نویسندگان
چکیده
منابع مشابه
Generation and Evaluation of Monoclonal Autoantibodies in Systemic Lupus Erythematosis
Three murine monoclonal anti-ssDNA autoantibodies have been produced by fusion of spleen cells from NZB/NZW F1 hybrid mice with Sp2/0-Ag 14 murine myeloma cells. Specificity studies characterizing monoclonal anti - ssDNA autoantibodies revealed binding patterns for nucleotides. Clone E-4-1 showed preferential binding with mono-di-and polyguanosine. Clones E-4-4 and E-11-1 specitically bound mon...
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Hybridomas are cells that have been engineered to produce a desired antibody in large amounts. To produce monoclonal antibodies, Bcells are removed from the spleen of an animal that has been challenged with the relevant antigen. These B-cells are then fused with myeloma tumor cells that can grow indefinitely in culture (myeloma is a B-cell cancer). This fusion is performed by making the cell me...
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Production of monoclonal antibodies requires immortalization of splenocytes by somatic fusion to a myeloma cell line partner (hybridomas). Although hybridomas can be immortal, they may depend on a feeder cell layer and may be genetically unstable. Since the inception of hybridoma technology, efforts to improve efficiency and stability of monoclonal antibody-producing cell lines have not brought...
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Description: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today truly an essential publication for researchers in all fields ...
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A hybridoma-derived monoclonal antibody. produced by immunization with the Burkitt’s tumor-derived B-Iymphoblastoid cell line. B35M. was previously shown to detect a 68.000 dalton surface membrane protein. B12. on the surface of peripheral blood B cells. which is absent from thymocytes. T cells. and granulocytes. In this study. we investigated the expression and distribution of B12 on benign an...
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ژورنال
عنوان ژورنال: Juntendo Medical Journal
سال: 1983
ISSN: 0022-6769,2188-2134
DOI: 10.14789/pjmj.29.322